ELISA Complement Activity Analysis

Complement Animal Measurement In Vivo and In Vitro

Heparin Plasma (10-15 IU/ml Heparin) Hirudin Plasma (50 μg/ml Hirudin) Serum EDTA or citrate plasma cannot be used.

If testing is not to be performed immediately, aliquot samples into appropriate volumes and store frozen at –70°C For previously frozen specimens, thaw samples in cold water. After thawing place samples in ice bath until use.

Bovine, Chicken, Dog, Goat, Guinea Pig, Horse, Mini Pig, Pig, Rabbit, Rat, Sheep and Turkey

The Pan-Specific C3 Reagent Kit represents a novel approach to fill the gap of animal-specific Complement ELISAs. The C3 Complement Matrix (CCM) and the C3 Converter Reagent (CCR) in the kit convert the activity of C3 in the animal specimen to human SC5b-9 that is detectable with the SC5b-9 Plus EIA kit. Thus the method allows sensitive and quantitative measurement of C3 in animal plasma or serum, which, to the extent C3 had been consumed prior to the assay, also provides a measure of prior Complement activation. The method expands the methodical arsenal of Complement analysis in animals, enabling, among many applications, npreclinical immune toxicology testing of C-mediated (pseudoallergic) adverse drug effects.

The PS-C3 method is a three-step procedure. In the first step animals are treated with, or anima sera or plasma samples are incubated with the test drugs or agents or device to explore possible Complement activation. Samples are collected at appropriate times for the second step: conversion of animal C3 to human SC5b-9. In the third step SC5b-9 is measured using the human SC5b-9 Plus ELISA kit (Cat. No. A029).