ELISA Complement Activity Analysis

Quantification of the SC5b-9 complex

Serum, EDTA plasma, spinal fluid or other biological fluids

The proper collection and storage of specimens is essential since SC5b-9 may be generated in improperly handled specimens. Serum or EDTA plasma specimens should be collected aseptically using standard techniques. They should be tested immediately or stored at 4 ºC or on ice until assayed. This should not exceed four hours. For longer-term storage freeze at -70 ºC. Plasma concentrations better reflect in vivo concentrations in comparison to serum concentrations.

Serum 334 - 1672 ng/ml EDTA Plasma 127 - 303 ng/ml

Human, cynomolgus macaque, baboon, rhesus macaque

Monoclonal mouse-antibody, specifically binds SC5b-9.

The SC5b-9 enzyme immunoassay measures the amount of SC5b-9 present in human plasma, serum and other biological or experimental samples. The Terminal Complement Complex (TCC, SC5b-9) is generated by the assembly of C5 through C9 as a consequence of activation of the complement system by either the classical, lectin or alternative pathway. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates the irreversible target cell membrane damage associated with complement activation.