Purified Complement Proteins

Purified human complement component C3

The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/- 0.52 mg/ml.

Specifications:Concentration: 1.0 mg/mlVolume/Vial: 250 µl/vialStorage: at or below -70°CPurity: > 95%Buffer: PBS

Functional Testing: All Quidel complement components are tested for functional activity in a standard lytic or applicable functional assay and for biochemical purity by SDS- polyacrylamide gel electrophoresis. Please refer to the Certificate of Analysis for lot specific information on functional titer.

Applications:Quidel"s purified human complement components are useful in a wide variety of immunochemical and research applications.

References:

• R. Marks, Hindmarsh, E., Complement activation occurs on subendothelial extracellular matrix in vitro and is initiated by retraction or removal of overlying endothelial cells, J Immunol 160:6128-6136, 1998.